Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. HTRF-compatible SpectraMax Multi-Mode Readers. 7°C and the bottom to 36. The system provides automation for up to 50 microplates for easy walkaway automation. These systems are hybrids that allow you to choose between. Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. Figure 1. Visible absorbance -. 0A-Sunnyvale, CA-Made in USAWe specialize in many of the Molecular Devices microplate readers, washers, and stackers including the SpectraMax®, VersaMax™, Gemini™, FlexStation™, AquaMax®, and StackMax® lines. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. 4: Test tube cover. 400×320×350 mm. Imaging System Giles Scientific Biomic V3 Automatic Plate. With three modes of detection for UV/Vis absorbance, fluorescence and luminescence, the SpectraMax®. Absorbance values were read on the SpectraMax iD5 reader using a preconfigured BCA assay protocol (the SpectraMax iD3 reader is also suitable for these assays, as it has the same absorbance performance as the SpectraMax iD5 reader). the washer with a final aspirate height of 4. 315 can't find zero order. The SpectraMax 190 reader can be integrated with Molecular . Versa. The Bauer Core Facility at Harvard University offers of variety of plate-reader choices to its users (mostly from Molecular Devices), and the SpectraMax® L is preferred for luminescence rather than the core’s multimode readers. 1. The StakMax® Microplate Handling System is a powerful, compact, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers for improved process flow and higher throughput. ®Plate chamber design of the SpectraMax category 1 microplate readers. Expand the Port (COM & LPT) Right click on the USB Serial Port (COMx) and select Properties. Step 4: Incubate at room temperature for 1 hour, protected from light. UV/Vis spectrophotometer & microplate reader with built-in cuvette port. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. Step 2. Unplug the power cable from the back of the instrument. Microplate Readers. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Step 1: Turn on your microplate reader. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. (1) Repertoires of SARS-CoV-2 epitopes targeted by antibodies vary according to severity of COVID-19 Virulence December 31, 2022 David J. 0077 OD 0. How to import latest protocol revisions into SoftMax Pro Protocol Library; Protocols for the ABS2 Validation Plate. Select the Driver tab. Absorbance was read at 590 nm on the SpectraMax ABS Plus Microplate Reader. Successful targets include calcium channels, chemokine receptors, and transiently transfected receptors. What plates do you recommend for SpectraMax Plate Readers? Luminescence: Solid white plates for top reads and white plates with clear bottoms for. 425 or. 9. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. EarlyTox Live/Dead Assay Kit (Molecular Devices cat. That’s why Molecular Devices offers the following services: Installation qualification (IQ), operational qualification (OQ), preventive maintenance (PM), and repair coverage. Increase productivity with patented microplate reader technologies. 1. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. 561. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. Filter 320/100 (available from Molecular Devices) Filter 616/10 (Molecular Devices cat. VIS absorbance detection. This assay kit is optimized for SpectraMax microplate readers from Molecular Devices, with a pre. This process should take about 3 - 5 minutes. FLIPR Calcium 4 Assay Kit. FilterMax F3 and F5 Multi-Mode Microplate Readers are filter-based microplate readers offering fluorescence polarization, time-resolved fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. If flexibility becomes important in a growing lab supporting a variety of projects, the decision might come down to whether. The new assay will complement Molecular Devices’ workflow solution for cell line development by offering a 10-minute titer assay that can be run on the company’s SpectraMax series of microplate. the iD5 reader’s filter system. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Uncontrolled Copy for Training and Reference Purposes Only . The SpectraMax® i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one systemPrimary considerations when choosing a microplate reader are throughput and flexibility. The small footprint and touchscreen control allows for easy laboratory setup with minimal investment of time, cost, and effort. Part Number Description 1-2100-0548 USB computer connection cable, 3 meter (9. The touchscreen interface provides integrated instrument control, data display, and the ability to export results for statistical data analysis. Luminescence was read every five minutes for five hours with three seconds of orbital shaking before each read. Step 1: Open the SoftMax Pro IMAP FP protocol. The SpectraMax® QuickDrop™ UV-Vis Spectrophotometer quantifies very small amounts of DNA, RNA, oligos, and proteins. Leveraging this novel technology, researchers can: Add western blot detection to their microplate reader within 2 minutes. The plate was covered and mixed on an orbital shaker for five minutes and then placed in a SpectraMax i3x reader and mixed. Here, we demonstrate that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. 40266 Email: drugdiscoverytech@lifetech. 0 . With optimized reagents, option to operate the. Microplate Readers. The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular biology and immunoassays. Vmax® plate reader specifications. Page 1 SpectraMax M2/M2 user guide ® A MULTI-DETECTION MICROPLATE READER WITH TWO-MODE CUVETTE PORT. Optimize wavelengths-protocols from Molecular Devices are on computer desktop. PathCheck sensor corrects optical pathlength & pipetting variation. 5: Schematic of the back panel of a reader. Learn more about how absorbance is measured, and some key applications that utilize absorbance. Page 1 of 8 . The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. SoftMax Pro GxP Software. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. Important things to consider when purchasing a multimode microplate reader. Expand the Port (COM & LPT) Right click on the USB Serial Port (COMx) and select Properties. (a) Chemical structures of 1 (HADP) and its analogues 2–4. generation SpectraTest ABS2 Absorbance Validation Plate. Molecular Devices launches custom organoid line expansion service using unique bioprocess technology for high-throughput applications. This multi plate reader is excellent to work with for checking the U. Restart the system. FAST AND ACCURATE METERTECH Microplate Reader $3,650. washer including mechanical, electrical, and optical components to verify operating functions are in accordance to the Molecular Devices product operational specifications. Two. Use preconfigured wash, soak, and aspiration protocols or create your own multi-step protocol using the simple touchscreen interface. Easy-to-use touchscreen allows you to quickly set up your experiments, use preconfigured analysis methods, and view tutorial videos. Powerful, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers. Five-mode microplate reader with automatic filter identification and western blot capability The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence. Select Start>Control panel>Device Manager. 9. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. On the back, in the right hand top corner, there. These models can no longer be repaired. Absorbance Plate Reader Comparison Chart. A: adipocytes; B: fibroblasts; C: no cells (uptake reagent only). Some plate readers handle label-free cell-based assays, such as PerkinElmer’s EnSpire® multimode reader, which in addition to absorbance, luminescence, fluorescence and AlphaLISA also supports Corning’s Epic® technology. To update the driver. The plate was incubated at room temperature for 50 minutes or 2. SpectraMax iD3 Multi-Mode Microplate Reader (Molecular Devices cat. Read one sample or up to. Vmax® plate reader specifications. Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. The VersaMax Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). SpectraDrop High-Throughput Screening (HTS) Kit. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. An absorbance plate reader offers higher throughput and can measure the absorbance of samples in microplates (typically 96-well or even 384-well) by sending light through each well. Devices Absorbance Microplate Readers. Molecular Devices, LLC Optical System: Inquire; Measuring Time: White light - 96-well/ 384 well microplate: <2 min Fluorescence - 96-well/ 384 well microplate: <8 min. and California)Our specialized plate readers for absorbance, fluorescence, and luminescence detection provide optimal sensitivity. After centrifugation, the plate was immediately read on the M5e and i3x readers using the instrument settings listed in Table 1. Step 2: Add sample to well – antigen within the sample binds to the capture antibody. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. Plate reader. We would like to show you a description here but the site won’t allow us. The FilterMax™ F3 features absorbance (visible), fluorescence intensity - FI (top reading), and glow luminescence for 96- to 384-well plates. The CellTiter-Glo assay from Promega uses luciferase. Microplates for applications that require high sample recovery rates. Test up to 3. ; Page 2 Molecular Devices equipment, software, reagents, and consumables. SpectraTest ABS2 Absorbance Validation Plate SpectraMax iD3, iD5, i3x, i3, M2, M2e, M3, M4, M5, M5e,. When connecting for the first time to either the SpectraMax L reader or StakMax system. 0. The Bauer Core Facility at Harvard University offers of variety of plate-reader choices to its users (mostly from Molecular Devices), and the SpectraMax® L is. 747. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. Urban myths of microplate readers. Multimode microplate readers offer flexibility, and ease of use. If the computer is connected to the Internet: Select Search Automatically for. Page 1 ™ VERSAmax Tunable Microplate Reader Operator’s Manual for Instruments with Serial Numbers Beginning with BN Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0084 Rev. 과 같은 Parameter를 검사하여 평가할 수 있습니다. adipocytes: insulin dose-response. The instrument supports the following read modes:. VMax . Hold down the green power button on the front of the instrument for 5 seconds or until the instrument power down. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. With three modes of detection for full spectrum absorbance, fluorescence and luminescence, the SpectraMax® Mini reader . 340 PC384. Use all black plate. together with a Molecular Devices plate. In . The SpectraMax ABS Plus reader utilizes Molecular . Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. #iD3) Methods. The system comes with a single plate loading adapter and a choice of magazines for 20, 40 or 50 SBS-standard 96- and 384-well. Recertification service. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature dependency using the patented. Flexibility to read a variety of plate formats. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. Devices PathCheck® Sensor—the only patented technology available that measures the depth (optical pathlength) ofWe would like to show you a description here but the site won’t allow us. 100 μL of each endotoxin standard was added to triplicate wells of an LAL Reagent Grade Multi-well Plate. 0112-0127C 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 9 ComputerIntegration 11 InstrumentCovers 11 FluidicsModule 12Plate reader instructions Computer. Monthly Plate Verification Check (Absorbance Validation Plate) Note: Monthly Plate Verification Check is maintained by Quality Control. A. 0) at 37 °C, fluorescence intensity was recorded on a Spectramax M5 Multimode plate reader. The microplate (containing standards) was incubated at 37ºC in the preheated SpectraMax ABS Plus reader for 10 minutes. This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in writing. The reader measures both flat and round 96-well microplates and. Tests every subsystem of the reader . 9. Weight. Fig 2: Enzymatic response of synthetic compounds. First, as mentioned before, the walkaway time significantly. IMAP® Technology from Molecular Devices enables rapid, homogeneous, and non-radioactive assay of kinases, phosphatases, and phosphodiesterases and is suited for both assay development and high-throughput screening. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. Dual monochromators increase performance and assay flexibility. Get any of the iD series microplate readers with up to 25% discount off the current list price in their basic configuration and receive a two-year warranty for commercial or a three-year warranty for Academic customers. Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. 0) OD (0. The Beacon Melamine Plate Kit is a competitive enzyme-linked immunosorbent assay (ELISA) for the quantitative analysis of melamine in contaminated samples. 1. SpectraMax Microplate Readers. The VMax® Microplate Reader is capable of reading up to two wavelengths at a time in the visible wavelength spectrum (400nm–750nm), with 4 filter choices included at time of purchase. The AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates. The adapter is required for optimum performance with standard 96-well and 384-. Uncontrolled Copy for Training and Reference Purposes Only . TECHNICAL NOTE. Superior optical system includes a xenon flash lamp and features. 2. FlexStation 3 Multimode Microplate Reader (Molecular Devices Cat. Cells were thawed and propagated according to the recommended method specified by the supplier. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. A kinetic plate read was initiated at time zero. 2. Chapter 1: Introduction The SpectraMax® iD3 Multi-Mode Microplate Reader from Molecular Devices is a monochromator-based, multi-mode plate reader. #4TI-0214) UltraVision Plate 96 (Brooks Life. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). , prior to compound addition) from the cells. Detection cartridge modularity allows for easy addition of new read modes within minutes without. As Glazer explains, “Our readers have software that analyzes data as it’s acquired from a plate—doing both at the same time. No accessories are needed and SoftMax® Pro Software. The former have been the traditional domain of plate readers and great strides have been. Additional high-quality filters to. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set ofRead plate standard and mix. * Filters (diameter) or optics shown as center wavelength/bandwidth (nm) & dichroic mirrors shown as cutoff wavelength (nm); Contact Molecular Devices for information on ordering filters & dichroic mirrors suitable with the microplate readers listed above. The computer controlling the plate reader runs Windows XP. Molecular Devices' microplate readers. Other readers with cell imaging include PerkinElmer’s EnSight™ Multimode Plate Reader and Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader. 1 up to 3600 sec. SAN JOSE, Calif. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11. One plate carrier adapter is provided with the instrument. ORDER. Purchase a new SpectraMax Multi-Mode Microplate Reader and. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. The plate reader optics are tunable for absorbance,. Cellular Imaging Systems: Molecular Devices offers various imaging solutions for your research needs. . FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode Detection Platform. GxP. 5577 UK +44. Do you want to learn how to use the FilterMax F3 and F5 Multi-Mode Microplate Readers for your research? Download the user guide and find out how to install, operate, and troubleshoot the instruments. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. A standard spectrophotometer measures the absorbance of one sample at a time. for SoftMax Pro Software. The automated ELISA workflow has two positive outcomes. Microplates for Molecular Devices Microplate Readers – Photometric Accuracy Fluorescence Intensity Luminescence Intensity (0. Select Start>Control panel>Device Manager. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. The procedures for these tests are outlined in the instrument user manual. If it looks fine, then proceed. Built-in Help function answers your most frequently asked questions. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax ® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. Devices’ StakMax® Microplate Stacker in a matter of minutes and . #4TI-0655) Microplates. The adapter is required for optimum performa nce with standard 96-well and 384-. It is more inert and reduces compound interference. #P7589 or P11496) • Black 96-well plate (Greiner Bio-One, cat. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. com #THX1138 Introduction The proteasome is a massive protein complex inside all eukaryotic cells (and some bacteria) that removes. These lab workhorses are used in drug discovery. 190. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. High-precision 9 channel system; filter-less with up to 6 wavelengths. We would like to show you a description here but the site won’t allow us. 0%, raw data units = counts/sec. M5. Wide photometric range Temperature-regulated Thermal control and vertical shake Microsoft Windows-based software Automatic mixing Five-second read per plate Photometric and kinetic analysis mode The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. begin reading microplates with seven mouse clicks. 3T3 L1 adipocytes were plated at 50,000 cells/well in 100 µL of DMEM/FBS in a 96-well plate and incubated at 37°C for five hours,Lower variance: Normalize your plate reader assay data to the number of cells in each well. Patented pathlength correction for better absorbance accuracy Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature dependency using the patented PathCheck® Sensor technology. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. Use the 1. Recombinant protein expression Nucleocapsid. Chapter 1: Introduction The SpectraMax iD3 Multi-Mode Microplate Reader from Molecular Devices is a monochromator-based, multi-mode plate reader. They are widely used in research, drug discovery, [1] bioassay validation, quality control and manufacturing processes in the pharmaceutical. 1. Supplier: Molecular Devices The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes. Cellular Imaging Systems. V. 2 GxP Software is our most secure software to help you achieve full FDA 21 CFR Part 11 compliance with streamlined workflows to ensure data integrity. Cellular Imaging Systems. Flexibility to read a variety of plate formats. A microplate reader with fluorescence intensity (FI) detection uses a light source, usually a Xenon flash lamp or LED, to excite a fluorophore (fluorescent molecule) at a particular wavelength. ABS2, FL1, and LM1 Validation Plates sent to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, an then returned with a new certificate of calibration. What are the physical and environmental operating specifications for SpectraMax UV/Vis and Gemini plate readers? (With extra info on backup power UPS choices). Clone Screening. SoftMax Pro Software. SpectraTest ABS2 Absorbance Validation Plate SpectraMax iD3, iD5, i3x, i3, M2, M2e, M3, M4, M5, M5e,. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. 2. and fluorescence microplate readers—. For researchers working in GLP or GMP laboratories, achieve full GxP compliance with our turnkey solution:. M5. Shop now. SpectraMax iD3. ®This document is provided to customers who have purchased Molecular Devices, Inc. PURPOSE. Measure more accurately. One plate carrier adapter is provided with the instrument. SpectraMax Readers have the most complete level of product validation and compliance. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. The calculated LOQ values were 0. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great. Molecular Devices also offers Digital IQ/OQ/PM. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide 0112-0102 Rev. Complete plate reader solution for all your research needs For over 40 years, we have partnered with scientists to expand the boundaries of their research. 58% for the detection module and 2. For more sample throughput, the system can be easily integrated into a full roboticThe SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Our 15 years of experience means we understand these instruments as well as the manufacturer, and it allows us to provide top-quality service. 1 up to 3600 sec. com Setup for Z’-LYTE® Assay on SpectraMax® M5/M5e. 020 OD 2. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. 18 kg. #I3X), equipped with the following: HTRF Detection Cartridge (Molecular Devices cat. The cellular imaging systems Molecular. 306 plate air cal. 2. Evaluation of BioTek's Synergy H4 Plate Reader for Fluorescent Detection. The advantage of using labeled probes is detection specificity. FilterMax F3 and F5 Multi-Mode Microplate Readers are filter-based microplate readers offering fluorescence polarization, time-resolved fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. Instrument settings can be saved as a protocol file. Read the plate on the SpectraMax M5, M5e, or FlexStation 3 reader using the optimized settings shown above. For more sample throughput, the system can be easily integrated into a full robotic The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter. SoftMax® Pro 7. 00. 2. Select the Driver tab and click Update Driver. View the Molecular Devices website. 10,700. #S1207. Wide photometric range Temperature-regulated Thermal control and vertical shake Microsoft Windows-based software Automatic mixing Five-second read per plate Photometric and kinetic analysis mode The Molecular. PURPOSE. Only SoftMax Pro 5 or older versions. Molecular Devices' microplate readers. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. Learn more about their features and specifications in. The Spectratest® Abs1 plate, which is used for validationof Molecular Devices monochromator based readers, cannot be used to validate the Emax/Vmax readers. Read times. 2 GxP Software is our most secure software to help you achieve full FDA 21 CFR Part 11 compliance with streamlined workflows to ensure data integrity. Turn on Plate Reader and wait for it to complete its start-up routine. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. 150 μL of MTT solvent was then added to each well to solubilize the formazan product, and the plate was placed on a shaker for 15 minutes. Single- and multimode microplate readers are useful for very broad range of applications including ELISA, kinetics and high throughput screening assays. Get the most out of SoftMax Pro. Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part #0112-0140 Rev. Instruments are configurable for the assay-specific detection modes and performance your laboratory needs. To maintain confidence in the standards, Molecular Devices recommends having validation plates recertified at one-to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, and Luminescence is the emission of light by a substance as a result of a chemical reaction called chemiluminescence or an enzymatic reaction called bioluminescence. The low profile increases robotic stack capacity and storage. Wait 20 seconds. Other features and benefits include: Eliminate changing out filters. Fluorescence Microplate Reader Comparison Chart. Next generation SpectraMax® absorbance microplate readers. 9000-0756. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. Flexibility to read a variety of plate formats - SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. Getting to know your new SpectraMax iD3 Multi-Mode Microplate Reader View a brief overview of the features and benefits of your new SpectraMax iD3 Multi-Mode. View the LabX ads below to find Molecular Devices SpectraMAX plate readers for sale and. If the computer is connected to the Internet: Select Search Automatically for updated driver software. Shop now. absorbance readers. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. Preventive Maintenance (PM) Each plate reader or washer is calibrated, inspected, and lubricated— any potential issues are. Eliminate time-dependent substrate addition steps. Instrument settings can be saved as a protocol file. SpectraMax readers support page; Analyst and FlexStation reader support page; Plate handlers and washers support page; We are continuously adding material to the knowledge base. If no sound is heard during initialization, contact technical support. Exploring misconceptions and understanding the technologies. This five-mode microplate reader with automatic filter identification and western blot capability is the complete. The plate was placed on a shaker for 30 seconds and then incubated for 3 hours at 37°C, 5% CO 2. For the name of the nearest authorized Molecular Devices Corporation service facility please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. Molecular Devices is one of the leading provider of high-performance bioanalytical measurement solutions for life science research, pharmaceutical and biotherapeutic. Step 4: Add detection antibody – Enzyme-conjugated detection antibody binds to a second site on the. HTRF-compatible SpectraMax Multi-Mode Readers. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry-leading SoftMax Pro. 304 signal level saturation. F3/F5 Multi Mode Microplate Readers. Small but mighty absorbance plate reader •ffordable monochromator-based plate reader with linearity A of up to 3 OD • orks with 96- and 384-well plates as well as cuvettes W. ISM Equipment Classification (Group 1, Class A) This equipment is. Fibroblasts vs. 0. DOCUMENTATION. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol ‘NanoOrange’. It this case, the EMax Plus Microplate Reader was the default. Turn on Plate Reader and wait for it to complete its start-up routine. 9. Instrument settings can be saved as a protocol file. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. Dual monochromators increase performance and assay flexibility. Dimensions: 128mm (L) x 86mm (W) x 10mm (H). Luminescence detection typically offers greater sensitivity than absorbance or fluorescence detection. FLIPR Tetra fluorometric imaging plate reader (Molecular Devices, Inc) with EMCCD Camera and 440–480 nm and 515–575 nm emission filters; Methods Cell Preparation. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. #6590-0108) Fluorescence detection using the SpectraMax iD5 reader’s monochromators for wavelength selection was compared to that using a filter pair specific for the Alexa594 tracer. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. Monday to Friday 7am - 5pm Pacific Time. 305 reference level saturation. Monthly Plate Verification Check (Absorbance Validation Plate) Note: Monthly Plate Verification Check is maintained by Quality Control. #0200-7009) Optimal instrument settings for ValitaTiter (P/N VAL003 or VAL013, left table) and ValitaTiter Plus (P/N VAL004 or VAL014, right table) assays on the SpectraMax i3x reader are shown below. 1. 0 . The Versamax Microplate Reader is built around a grating monochromator. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. Tests every subsystem of the reader or . Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. D SpectraMax ® M2 SpectraMax ® M2 e Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. Wide photometric range Temperature-regulated Thermal control and vertical shake Microsoft Windows-based software Automatic mixing Five-second read per plate Photometric and kinetic analysis mode The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader.